Prima RNApols™ ExTend Kit
Long-template mRNA synthesis with Prima RNApols™ ExTend Kit
Prima RNApols™ ExTend is an innovative RNA polymerase engineered to address challenges in the in vitro transcription (IVT) manufacturing of mRNA.
SEE DETAILS
Prima RNApols™ ExTend Kit
Contains the following for 50 IVT reactions:
• 5,000 U RNA Polymerase (100 µL)
• 4, 5x Reaction Buffer (100 µL)
• 2 kb Linearized DNA Template (25 µL)
For Research Use Only (RUO)
$449.00
Key Features & Benefits
High-Quality, High-Purity mRNA
Generates long-template mRNA with superior integrity compared to T7 polymerase.
Ultra-Low dsRNA
Minimizes unwanted byproducts, reducing immune response risks and improving the safety and effectiveness of mRNA therapeutics and vaccines.
Maximized Yield with Less Input
Increases mRNA output while using less DNA template, lowering costs and streamlining the manufacturing process.
Compatibility with Various mRNA Structures
Supports diverse mRNA modalities and modified nucleotides.
PRODUCT DETAILS
Prima RNApols™ ExTend Kit
Contains the following for 50 IVT reactions:
• 5,000 U RNA Polymerase (100 µL)
• 4, 5x Reaction Buffer (100 µL)
• 2 kb Linearized DNA Template (25 µL)
For Research Use Only (RUO)
Coming soon: Prima RNApols™ ExTend for GMP mRNA manufacturing
Seamlessly transition from discovery to clinical applications.
Total mRNA yield and integrity were measured on an Agilent Fragment Analyzer capillary electrophoresis system across all RNA sizes. The target yield was determined by quantifying the 5kb-sized peak. Integrity was calculated as the amount of the target RNA species as a percentage of total RNA. dsRNA levels were measured for equivalent amounts of total RNA synthesized in IVT reactions by immunoblotting with the J2 monoclonal antibody and quantitating spot intensities with ImageJ software.
Total mRNA yield was measured on an Agilent Fragment Analyzer capillary electrophoresis system across all RNA sizes. The target yield was determined by quantitating the 5kb-sized peak.
dsRNA levels were measured for equivalent amounts of total RNA synthesized in IVT reactions by ELISA using the SCICONSTM dsRNA ELISA Kit (based on the J2 monoclonal antibody).
What is the Prima RNApols™ ExTend promoter sequence?
TTGATTAATTAAC CCACACTATAGGG
Will the Prima RNApols™ ExTend kit work with the T7 promoter?
No, the Prima RNApols™ ExTend polymerase is only compatible with the Prima ExTend promoter. This sequence must be incorporated into the DNA template of interest to successfully generate mRNA during IVT.
Will the Prima RNApols™ ExTend kit work with any transcription start site (TSS)?
Yes, however, there are certain TSS that Prima RNApols™ ExTend is ideally suited for.
Can I use my preferred cap analogue with this kit?
… ideally suited for post-translational capping [Leave off until we have the data?]
What is in the 5x Reaction Buffer?
A buffer system that includes traditional IVT reaction components.
Can I use more or less than 8nM DNA template?
Yes. 8nM is suggested as it results in consistently high yields of high-quality mRNA product across different template lengths. Using more or less DNA template will still work but may affect yield and/or quality of your end product.
Can I use a different reaction buffer than the one provided in the kit?
Yes, but the 5x Reaction Buffer in the kit has been optimized for Prima RNApols™ ExTend.
How do I incorporate the Prima RNApols™ ExTend polymerase promoter into my DNA template?
By PCR. Use a forward primer with the Prima RNApols™ ExTend sequence above with a 20 nt overlap of your template’s 5’ end, and a reverse complement primer complementary to your template’s 3’ end.
How can the yield of RNA be maximized when using Prima RNApols™ ExTend polymerase?
Increase the concentration of IVT components.
Do I need to purify the RNA before transfection into mammalian cells or measuring translation in an in vitro system?
It’s recommended to purify the RNA to remove IVT components.
Will the Prima RNApols™ ExTend polymerase work on both PCR and plasmid-based templates?
Yes, it will work on both types of DNA templates.
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