mRNA MANUFACTURING SOLUTIONS: REDEFINING MANUFACTURABILITY FOR mRNA-BASED THERAPIES
Primrose Bio provides comprehensive solutions for low cost and high-quality mRNA manufacturing powered by Prima RNApols™ – the highest performing RNA polymerases available.
Purchase Prima RNApols
Custom RNApol Evaluation
Tailored RNApol Improvement
mRNA Analytical Solutions
IVT Process Development
RNApols Manufacturing
Prima RNApols Key Benefits
By outperforming the industry-standard T7 RNA polymerase, our Prima RNApols improve the cost and quality profiles of pharmaceutical mRNA manufacturing across a range of different DNA template sequences and lengths, as well as a variety of capping chemistries, modified nucleotides and IVT conditions.
Purchase Prima RNApols
Source our superior Prima RNApols designed for long templates and ultra-low dsRNA in early 2025. Available in reagent-grade kits and off-the-shelf enzymes for GMP mRNA production.
- Prima RNApols™ ExTend kit for use with long DNA templates
- Ultra-low dsRNA coming soon
Custom RNApol Evaluation
Primrose Bio has developed a panel of over 20 different RNA polymerases. We screen this panel to identify enzymes that deliver high IVT performance using your materials and to the desired Key Performance Indicators (KPIs). These include yield, dsRNA, integrity, capping efficiency and more.
- Leverage the power of the panel
- Identify an RNApol suited for your product and process
Tailored RNApol Improvement
Enzymes are flexible biocatalysts that can be evolved to perform with the substrates and conditions they are required to operate in. Primrose Bio has developed an industry leading, ultra-high-throughput enzyme evolution workflow to tailor RNA polymerases for mRNA manufacturing. We improve RNApols from our panel efficiently and rapidly to meet your IVT KPIs. We support this effort with high quality enzyme and mRNA analytics from our best-in-class analytical sciences team.
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mRNA Analytical Solutions
Our analytical sciences team supports these offerings with a range of orthogonal analytical approaches for deep characterization of mRNA. We also developed unique analytical assays well suited for screening that are available to you.
- High-quality characterization of mRNA
- High-throughput analytics for screening
Purity of mRNA Fragment Analyzer, CE-SDS, CGE-SDS, HPLC
Structural Characterization of mRNA 5’-Capping Analysis by LC-MS, 3’-Poly-A Tail Analysis by LC-MS/HPLC/CE-SDS, CD for secondary structure
Translation Assessment Cell-Free Expression Analysis, Protein Characterization by Western Blot/ELISA/LC-MS
Residual Impurity Quantification dsRNA quantification, Endotoxin determination, Residual plasmid DNA, Residual Host-Cell Protein, Residual NTP and Capping Reagents
IVT Process Development
We leverage our team’s experience in both upstream and downstream process development. We rely on a systematic design of experiments (DoE) approach that is supported by a high degree of automation. Our goal is to develop an IVT process that maximizes the performance of the RNApols selected by you. This delivers the best possible outcome for your mRNA production.
RNApols Manufacturing
Starting in 2025, we will be fully equipped to supply custom Prima RNApols for GMP manufacturing to customers, providing industry-standard solutions for the most demanding applications.
100+
100+ PARTNERSHIPS IN INNOVATIVE PROTEIN AND NUCLEIC ACID-BASED THERAPEUTICS.